Instrument: NextSeq 500
Strategy: RNA-Seq
Source: TRANSCRIPTOMIC
Selection: cDNA
Layout: PAIRED
Construction protocol: RNA extraction was done using TRI Reagent [SIGMA] following manufacturer´s protocol. The precipitated RNAwas taken for library preparation. Libraries were constructed Stranded ligation-based, total-RNA libraries preparation was modified from (Engreitz et al., 2013) as described in Reddy et al 2018, Biorxiv.